Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-82834-2 targets ARL13B in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ARL13B fusion protein Ag12015 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosylation factor-like 13B |
| Calculated molecular weight | 48 kDa |
| GenBank accession number | BC094725 |
| Gene Symbol | ARL13B |
| Gene ID (NCBI) | 200894 |
| Conjugate | CoraLite® Plus 555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 554 nm / 570 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q3SXY8 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 555 ARL13B antibody CL555-82834-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |