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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-1376 cells using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    IF Staining of HT-1376 using 66732-1-Ig (same clone as 66732-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-1376 cells using ARNT/HIF-1 beta antibody (66732-1-Ig, Clone: 3B12C5 ) at dilution of 1:1300 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    FC experiment of HeLa using 66732-1-Ig (same clone as 66732-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ARNT/HIF-1 beta (66732-1-Ig, Clone:3B12C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

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ARNT/HIF-1 beta Monoclonal antibody, PBS Only

ARNT/HIF-1 beta Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 66732-1-PBS
Clone No.3B12C5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

ARNT, HIF-1 beta, HIF1B, 3B12C5, HIF1 beta

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66732-1-PBS targets ARNT/HIF-1 beta in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARNT/HIF-1 beta fusion protein Ag5507 相同性解析による交差性が予測される生物種
Full Name aryl hydrocarbon receptor nuclear translocator
Calculated molecular weight 416 aa, 46 kDa, 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC060838
Gene Symbol ARNT/HIF-1 beta
Gene ID (NCBI) 405
RRIDAB_2882082
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27540
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia