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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARNT/HIF-1 beta Monoclonal antibody

ARNT/HIF-1 beta Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3B12C5

Cat no : 66732-1-Ig

Synonyms

ARNT, HIF-1 beta, HIF1B, 3B12C5, HIF1 beta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    WB analysis using 66732-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    WB analysis using 66732-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    WB analysis using 66732-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HT-1376 cells using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    IF Staining of HT-1376 using 66732-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HT-1376 cells using ARNT/HIF-1 beta antibody (66732-1-Ig, Clone: 3B12C5 ) at dilution of 1:1300 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Flow cytometry (FC) experiment of HeLa cells using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    FC experiment of HeLa using 66732-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ARNT/HIF-1 beta (66732-1-Ig, Clone:3B12C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inCaco-2 cells, HeLa cells, HepG2 cells, HT-29 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHT-1376 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:650-1:2600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66732-1-Ig targets ARNT/HIF-1 beta in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARNT/HIF-1 beta fusion protein Ag5507 相同性解析による交差性が予測される生物種
Full Name aryl hydrocarbon receptor nuclear translocator
Calculated molecular weight 416 aa, 46 kDa, 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC060838
Gene symbol ARNT/HIF-1 beta
Gene ID (NCBI) 405
RRIDAB_2882082
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

Protocols

Product Specific Protocols
WB protocol for ARNT/HIF-1 beta antibody 66732-1-IgDownload protocol
IF protocol for ARNT/HIF-1 beta antibody 66732-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols