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CoraLite®594-conjugated ARNT,HIF1B Monoclonal antibody

ARNT,HIF1B Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3B12C5

Cat no : CL594-66732

Synonyms

ARNT, HIF1B, 3B12C5, HIF1 beta, HIF 1 beta

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated ARNT,HIF1B Monoclonal anti (CL594-66732)

    IF Staining of HepG2 using CL594-66732

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated ARNT,HIF1B antibody (CL594-66732, Clone: 3B12C5 ) at dilution of 1:200, CL488-Phalloidin (green).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated ARNT,HIF1B Monoclonal anti (CL594-66732)

    FC experiment of HeLa using CL594-66732

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ARNT,HIF1B (CL594-66732, Clone:3B12C5) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66732 targets ARNT,HIF1B in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARNT,HIF1B fusion protein Ag5507 相同性解析による交差性が予測される生物種
Full Name aryl hydrocarbon receptor nuclear translocator
Calculated molecular weight 416 aa, 46 kDa, 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC060838
Gene symbol ARNT/HIF-1 beta
Gene ID (NCBI) 405
RRIDAB_2883619
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

Protocols

Product Specific Protocols
IF protocol for CL594 ARNT,HIF1B antibody CL594-66732Download protocol
FC protocol for CL594 ARNT,HIF1B antibody CL594-66732Download protocol
Standard Protocols
Click here to view our Standard Protocols