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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARP3/ARP3B Monoclonal antibody (68439-1-Ig)

    WB analysis using 68439-1-Ig (same clone as 68439-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68439-1-Ig (ACTR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using ARP3/ARP3B Monoclonal antibody (68439-1-Ig)

    IHC staining of mouse spleen using 68439-1-Ig (same clone as 68439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 68439-1-Ig (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ARP3/ARP3B Monoclonal antibody (68439-1-Ig)

    IHC staining of mouse brain using 68439-1-Ig (same clone as 68439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68439-1-Ig (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using ARP3/ARP3B Monoclonal antibody (68439-1-Ig)

    IF Staining of HUVEC using 68439-1-Ig (same clone as 68439-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using ARP3/ARP3B antibody (68439-1-Ig, Clone: 1D12C7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

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ARP3/ARP3B Monoclonal antibody, PBS Only

ARP3/ARP3B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68439-1-PBS
Clone No.1D12C7

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Actin like protein 3, Actin related protein 3, ACTR3, ARP3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68439-1-PBS targets ARP3/ARP3B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARP3/ARP3B fusion protein Ag5017 相同性解析による交差性が予測される生物種
Full Name ARP3 actin-related protein 3 homolog (yeast)
Calculated molecular weight 418 aa 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC044590
Gene Symbol ARP3/ACTR3
Gene ID (NCBI) 10096
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61158
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.