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ARP3/ARP3B Monoclonal antibody

ARP3/ARP3B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68439-1-Ig
Clone No.1D12C7

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF/ICC, ELISA

Actin like protein 3, Actin related protein 3, ACTR3, ARP3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inCalu-3 cells, A549 cells, U2OS cells, HeLa cells, Human peripheral blood platelets, Pig lung tissue, Rabbit lung tissue, Rat lung tissue, Mouse lung tissue
Positive IHC detected inmouse brain tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68439-1-Ig targets ARP3/ARP3B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARP3/ARP3B fusion protein Ag5017 相同性解析による交差性が予測される生物種
Full Name ARP3 actin-related protein 3 homolog (yeast)
Calculated molecular weight 418 aa 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC044590
Gene Symbol ARP3/ACTR3
Gene ID (NCBI) 10096
RRIDAB_3085152
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61158
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

Protocols

Product Specific Protocols
WB protocol for ARP3/ARP3B antibody 68439-1-IgDownload protocol
IHC protocol for ARP3/ARP3B antibody 68439-1-IgDownload protocol
IF protocol for ARP3/ARP3B antibody 68439-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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