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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARP3/ARP3B Monoclonal antibody

ARP3/ARP3B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D12C7

Cat no : 68439-1-Ig

Synonyms

Actin like protein 3, Actin related protein 3, ACTR3, ARP3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARP3/ARP3B Monoclonal antibody (68439-1-Ig)

    WB analysis using 68439-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68439-1-Ig (ACTR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using ARP3/ARP3B Monoclonal antibody (68439-1-Ig)

    IHC staining of mouse spleen using 68439-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 68439-1-Ig (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ARP3/ARP3B Monoclonal antibody (68439-1-Ig)

    IHC staining of mouse brain using 68439-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68439-1-Ig (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using ARP3/ARP3B Monoclonal antibody (68439-1-Ig)

    IF Staining of HUVEC using 68439-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using ARP3/ARP3B antibody (68439-1-Ig, Clone: 1D12C7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inCalu-3 cells, A549 cells, U2OS cells, HeLa cells, Human peripheral blood platelets, Pig lung tissue, Rabbit lung tissue, Rat lung tissue, Mouse lung tissue
Positive IHC detected inmouse brain tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68439-1-Ig targets ARP3/ARP3B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARP3/ARP3B fusion protein Ag5017 相同性解析による交差性が予測される生物種
Full Name ARP3 actin-related protein 3 homolog (yeast)
Calculated molecular weight 418 aa 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC044590
Gene symbol ARP3/ACTR3
Gene ID (NCBI) 10096
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

Protocols

Product Specific Protocols
WB protocol for ARP3/ARP3B antibody 68439-1-IgDownload protocol
IHC protocol for ARP3/ARP3B antibody 68439-1-IgDownload protocol
IF protocol for ARP3/ARP3B antibody 68439-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols