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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ARP3/ARP3B Monoclonal antibody

ARP3/ARP3B Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D12C7

Cat no : CL488-68439

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite® Plus 488-conjugated ARP3/ARP3B Monoclona (CL488-68439)

    IF Staining of HUVEC using CL488-68439

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite® Plus 488 ARP3/ARP3B antibody (CL488-68439, Clone: 1D12C7 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68439 targets ARP3/ARP3B in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARP3/ARP3B fusion protein Ag5017 相同性解析による交差性が予測される生物種
Full Name ARP3 actin-related protein 3 homolog (yeast)
Calculated molecular weight 418 aa 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC044590
Gene symbol ARP3/ACTR3
Gene ID (NCBI) 10096
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ARP3/ARP3B antibody CL488-68439Download protocol
Standard Protocols
Click here to view our Standard Protocols