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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASAH1 Monoclonal antibody (67092-2-Ig)

    WB analysis using 67092-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67092-2-Ig (ASAH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ASAH1 Monoclonal antibody (67092-2-Ig)

    IF Staining of MCF-7 using 67092-2-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ASAH1 antibody (67092-2-Ig, Clone: 2B3C7 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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ASAH1 Monoclonal antibody

ASAH1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67092-2-Ig
Clone No.2B3C7

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

ASAH, Acid ceramidase subunit beta, Acid ceramidase subunit alpha, Acid ceramidase, AC

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig liver tissue, pig brain tissue, rabbit brain tissue, rat brain tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67092-2-Ig targets ASAH1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig, rabbit samples.

Tested Reactivity human, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASAH1 fusion protein Ag28645 相同性解析による交差性が予測される生物種
Full Name N-acylsphingosine amidohydrolase (acid ceramidase) 1
Calculated molecular weight 44 kDa
Observed molecular weight 44-55 kDa
GenBank accession numberBC016828
Gene Symbol ASAH1
Gene ID (NCBI) 427
RRIDAB_3085035
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13510
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.

Protocols

Product Specific Protocols
WB protocol for ASAH1 antibody 67092-2-IgDownload protocol
IF protocol for ASAH1 antibody 67092-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols