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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASAH1 Monoclonal antibody, PBS Only

ASAH1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B3C7

Cat no : 67092-2-PBS

Synonyms

ASAH, Acid ceramidase subunit beta, Acid ceramidase subunit alpha, Acid ceramidase, AC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASAH1 Monoclonal antibody (67092-2-Ig)

    WB analysis using 67092-2-Ig (same clone as 67092-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67092-2-Ig (ASAH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67092-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ASAH1 Monoclonal antibody (67092-2-Ig)

    IF Staining of MCF-7 using 67092-2-Ig (same clone as 67092-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ASAH1 antibody (67092-2-Ig, Clone: 2B3C7 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67092-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67092-2-PBS targets ASAH1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig, rabbit samples.

Tested Reactivity human, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASAH1 fusion protein Ag28645 相同性解析による交差性が予測される生物種
Full Name N-acylsphingosine amidohydrolase (acid ceramidase) 1
Calculated molecular weight 44 kDa
Observed molecular weight 44-55 kDa
GenBank accession numberBC016828
Gene symbol ASAH1
Gene ID (NCBI) 427
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.