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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ASF/SF2 Monoclonal antibody

ASF/SF2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A6C5

Cat no : CL488-66671

Synonyms

Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated ASF/SF2 Monoclonal a (CL488-66671)

    IF Staining of HepG2 using CL488-66671

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated ASF/SF2 antibody (CL488-66671, Clone: 1A6C5 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66671 targets ASF/SF2 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASF/SF2 fusion protein Ag3626 相同性解析による交差性が予測される生物種
Full Name splicing factor, arginine/serine-rich 1
Calculated molecular weight 248 aa, 28 kDa
Observed molecular weight 32 kDa, 28 kDa, 22 kDa
GenBank accession numberBC010264
Gene symbol ASF/SF2
Gene ID (NCBI) 6426
RRIDAB_2919354
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ASF/SF2 antibody CL488-66671Download protocol
Standard Protocols
Click here to view our Standard Protocols