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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated ASF1A Monoclonal antibody (CL594-66884)

    IF Staining of HepG2 using CL594-66884

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 ASF1A antibody (CL594-66884, Clone: 2D4A6 ) at dilution of 1:200, CL488-Phalloidin (green).

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CoraLite®594-conjugated ASF1A Monoclonal antibody

ASF1A Monoclonal Antibody for IF/ICC
Cat No. CL594-66884
Clone No.2D4A6

Host / Isotype

Mouse / IgG3

Reactivity

human, rat

Applications

IF/ICC

Synonyms

2D4A6, Anti-silencing function protein 1 homolog A, CCG1 interacting factor A, CCG1-interacting factor A, CGI 98

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66884 targets ASF1A in IF/ICC applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen ASF1A fusion protein Ag1233 相同性解析による交差性が予測される生物種
Full Name ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Calculated molecular weight 204 aa, 23 kDa
Observed molecular weight 21-23 kDa
GenBank accession numberBC010878
Gene Symbol ASF1A
Gene ID (NCBI) 25842
RRIDAB_2883631
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y294
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.

Protocols

Product Specific Protocols
IF protocol for CL594 ASF1A antibody CL594-66884Download protocol
Standard Protocols
Click here to view our Standard Protocols