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  • Featured Product
  • KD/KO Validated

ASGR1 Monoclonal antibody, PBS Only

ASGR1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G5F5

Cat no : 66692-1-PBS

Synonyms

ASGP R 1, ASGPR, ASGPR 1, ASGR1, asialoglycoprotein receptor 1, CLEC4H1, Hepatic lectin H1, HL 1, Hs.12056

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis of HepG2 using 66692-1-Ig (same clone as 66692-1-PBS)

    WB result of ASGR1 antibody (66692-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected HepG2 cells. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis using 66692-1-Ig (same clone as 66692-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis of L02 using 66692-1-Ig (same clone as 66692-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis of HepG2 using 66692-1-Ig (same clone as 66692-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse hepatocytes using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis of mouse hepatocytes using 66692-1-Ig (same clone as 66692-1-PBS)

    mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis of L02 using 66692-1-Ig (same clone as 66692-1-PBS)

    WB result of ASGR1 antibody (66692-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected L02 cells. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis of pig liver using 66692-1-Ig (same clone as 66692-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ASGR1 Monoclonal antibody (66692-1-Ig)

    IHC staining of human liver using 66692-1-Ig (same clone as 66692-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ASGR1 Monoclonal antibody (66692-1-Ig)

    IHC staining of human liver using 66692-1-Ig (same clone as 66692-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using ASGR1 Monoclonal antibody (66692-1-Ig)

    IF Staining of mouse liver using 66692-1-Ig (same clone as 66692-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using ASGR1 antibody (66692-1-Ig, Clone: 1G5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66692-1-PBS targets ASGR1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Pig, rat samples.

Tested Reactivity Human, Mouse, Pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASGR1 fusion protein Ag2310 相同性解析による交差性が予測される生物種
Full Name asialoglycoprotein receptor 1
Calculated molecular weight 291 aa, 33 kDa
Observed molecular weight 42-46 kDa
GenBank accession numberBC032130
Gene symbol ASGR1
Gene ID (NCBI) 432
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.