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ASGR1 Monoclonal antibody

ASGR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA, Blocking assay

Conjugate

Unconjugated

CloneNo.

1G5F5

Cat no : 66692-1-Ig

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis of HepG2 using 66692-1-Ig

    WB result of ASGR1 antibody (66692-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis using 66692-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis of L02 using 66692-1-Ig

    WB result of ASGR1 antibody (66692-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected L02 cells.

  • Western Blot (WB) analysis of L02 cells using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis of L02 using 66692-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis of HepG2 using 66692-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse hepatocytes using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis of mouse hepatocytes using 66692-1-Ig

    mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using ASGR1 Monoclonal antibody (66692-1-Ig)

    WB analysis of pig liver using 66692-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ASGR1 Monoclonal antibody (66692-1-Ig)

    IHC staining of human liver using 66692-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ASGR1 Monoclonal antibody (66692-1-Ig)

    IHC staining of human liver using 66692-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using ASGR1 Monoclonal antibody (66692-1-Ig)

    IF Staining of mouse liver using 66692-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using ASGR1 antibody (66692-1-Ig, Clone: 1G5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHuH-7 cells, L02 cells, HepG2 cells, pig liver tissue, mouse hepatocytes, HSC-T6 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66692-1-Ig targets ASGR1 in WB, IHC, IF-P, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASGR1 fusion protein Ag2310 相同性解析による交差性が予測される生物種
Full Name asialoglycoprotein receptor 1
Calculated molecular weight 291 aa, 33 kDa
Observed molecular weight 42-46 kDa
GenBank accession numberBC032130
Gene symbol ASGR1
Gene ID (NCBI) 432
RRIDAB_2882046
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

Protocols

Product Specific Protocols
WB protocol for ASGR1 antibody 66692-1-IgDownload protocol
IHC protocol for ASGR1 antibody 66692-1-IgDownload protocol
IF protocol for ASGR1 antibody 66692-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,Blocking assay

J Med Virol

The use of human iPSC-derived alveolar organoids to explore SARS-CoV-2 variant infections and host responses

Authors - Chaitanya Gandikota
View Article