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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using PE-conjugated ASGR1 Monoclonal antibody (PE-66692)

    IF Staining of rat liver using PE-66692

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using PE ASGR1 antibody (PE-66692, Clone: 1G5F5 ) at dilution of 1:100. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

PE-conjugated ASGR1 Monoclonal antibody

ASGR1 Monoclonal Antibody for IF-P
Cat No. PE-66692
Clone No.1G5F5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF-P

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  PE
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inrat liver tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-66692 targets ASGR1 in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASGR1 fusion protein Ag2310 相同性解析による交差性が予測される生物種
Full Name asialoglycoprotein receptor 1
Calculated molecular weight 291 aa, 33 kDa
Observed molecular weight 42-46 kDa
GenBank accession numberBC032130
Gene Symbol ASGR1
Gene ID (NCBI) 432
RRIDAB_3674012
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07306
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

Protocols

Product Specific Protocols
IF protocol for PE ASGR1 antibody PE-66692Download protocol
Standard Protocols
Click here to view our Standard Protocols