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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASGR2 Monoclonal antibody

ASGR2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A5C12

Cat no : 66831-1-Ig

Synonyms

ASGP R, ASGP R 2, ASGPR 2, ASGR2, asialoglycoprotein receptor 2, CLEC4H2, Hepatic lectin H2, HL 2, Hs.1259, L H2

Validation Data Gallery

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  • Western Blot (WB) analysis of SMMC-7721 cells using ASGR2 Monoclonal antibody (66831-1-Ig)

    WB analysis of SMMC-7721 using 66831-1-Ig

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 66831-1-Ig (ASGR2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using ASGR2 Monoclonal antibody (66831-1-Ig)

    WB analysis of HSC-T6 using 66831-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66831-1-Ig (ASGR2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inSMMC-7721 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66831-1-Ig targets ASGR2 in WB, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ASGR2 fusion protein Ag2037 相同性解析による交差性が予測される生物種
Full Name asialoglycoprotein receptor 2
Calculated molecular weight 39 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC017251
Gene symbol ASGR2
Gene ID (NCBI) 433
RRIDAB_2882174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

Protocols

Product Specific Protocols
WB protocol for ASGR2 antibody 66831-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols