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Validation Data Gallery

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  • Western Blot (WB) analysis of SMMC-7721 cells using ASGR2 Monoclonal antibody (66831-1-Ig)

    WB analysis of SMMC-7721 using 66831-1-Ig (same clone as 66831-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 66831-1-Ig (ASGR2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66831-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ASGR2 Monoclonal antibody (66831-1-Ig)

    WB analysis of HSC-T6 using 66831-1-Ig (same clone as 66831-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66831-1-Ig (ASGR2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66831-1-PBS in a different storage buffer formulation.

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ASGR2 Monoclonal antibody, PBS Only

ASGR2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66831-1-PBS
Clone No.2A5C12

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, Indirect ELISA

Synonyms

ASGP R, ASGP R 2, ASGPR 2, ASGR2, asialoglycoprotein receptor 2, CLEC4H2, Hepatic lectin H2, HL 2, Hs.1259, L H2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66831-1-PBS targets ASGR2 in WB, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ASGR2 fusion protein Ag2037 相同性解析による交差性が予測される生物種
Full Name asialoglycoprotein receptor 2
Calculated molecular weight 39 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC017251
Gene Symbol ASGR2
Gene ID (NCBI) 433
RRIDAB_2882174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07307
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.