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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ASNS Monoclonal antibody

ASNS Monoclonal Antibody for WB, IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C3D3

Cat no : CL488-68004

Synonyms

TS11, Glutamine-dependent asparagine synthetase, EC:6.3.5.4, Asparagine synthetase [glutamine-hydrolyzing], 1C3D3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated ASNS Monoclonal anti (CL488-68004)

    WB analysis using CL488-68004

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-68004 (ASNS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 488-conjugated ASNS Monoclonal anti (CL488-68004)

    IF Staining of SH-SY5Y using CL488-68004

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 ASNS antibody (CL488-68004, Clone: 1C3D3 ) at dilution of 1:200.

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Tested Applications

Positive WB detected inLNCaP cells, K-562 cells
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68004 targets ASNS in WB, IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASNS fusion protein Ag6817 相同性解析による交差性が予測される生物種
Full Name asparagine synthetase
Calculated molecular weight 64 kDa
Observed molecular weight 55-64 kDa
GenBank accession numberBC014621
Gene symbol ASNS
Gene ID (NCBI) 440
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 ASNS antibody CL488-68004Download protocol
IF protocol for CL Plus 488 ASNS antibody CL488-68004Download protocol
Standard Protocols
Click here to view our Standard Protocols