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Species Reactivity

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  • Featured Product
  • KD/KO Validated

ASS1 Polyclonal antibody

ASS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16210-1-AP

Synonyms

EC:6.3.4.5, ASS

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ASS1 Polyclonal antibody (16210-1-AP)

    WB analysis using 16210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ASS1 Polyclonal antibody (16210-1-AP)

    WB analysis using 16210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ASS1 Polyclonal antibody (16210-1-AP)

    WB analysis using 16210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ASS1 Polyclonal antibody (16210-1-AP)

    WB analysis using 16210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ASS1 Polyclonal antibody (16210-1-AP)

    WB analysis using 16210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using ASS1 Polyclonal antibody (16210-1-AP)

    WB analysis of rat kidney using 16210-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using ASS1 Polyclonal antibody (16210-1-AP)

    WB analysis of SMMC-7721 using 16210-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ASS1 Polyclonal antibody (16210-1-AP)

    IP experiment of HeLa using 16210-1-AP

    IP result of anti-ASS1 (IP:16210-1-AP, 4ug; Detection:16210-1-AP 1:1500) with HeLa cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using ASS1 Polyclonal antibody (16210-1-AP)

    IHC staining of human testis using 16210-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ASS1 Polyclonal antibody (16210-1-AP)

    IHC staining of human testis using 16210-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ASS1 Polyclonal antibody (16210-1-AP)

    IHC staining of human liver using 16210-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16210-1-AP (ASS1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ASS1 Polyclonal antibody (16210-1-AP)

    IHC staining of human liver using 16210-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16210-1-AP (ASS1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ASS1 Polyclonal antibody (16210-1-AP)

    IHC staining of human placenta using 16210-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ASS1 Polyclonal antibody (16210-1-AP)

    IHC staining of human placenta using 16210-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ASS1 Polyclonal antibody (16210-1-AP)

    IHC staining of human testis using 16210-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ASS1 Polyclonal antibody (16210-1-AP)

    IHC staining of human testis using 16210-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ASS1 Polyclonal antibody (16210-1-AP)

    IHC staining of human liver cancer using 16210-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ASS1 Polyclonal antibody (16210-1-AP)

    IHC staining of human liver cancer using 16210-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ASS1 Polyclonal antibody (16210-1-AP)

    IF Staining of HeLa using 16210-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ASS1 antibody (16210-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, rat kidney tissue, SMMC-7721 cells, Jurkat cells, mouse kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human liver tissue, human placenta tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16210-1-AP targets ASS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASS1 fusion protein Ag9200 相同性解析による交差性が予測される生物種
Full Name argininosuccinate synthetase 1
Calculated molecular weight 412 aa, 47 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC009243
Gene symbol ASS1
Gene ID (NCBI) 445
RRIDAB_2060466
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).

Protocols

Product Specific Protocols
WB protocol for ASS1 antibody 16210-1-APDownload protocol
IHC protocol for ASS1 antibody 16210-1-APDownload protocol
IF protocol for ASS1 antibody 16210-1-APDownload protocol
IP protocol for ASS1 antibody 16210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Immunity

Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.

Authors - Fangzhou Lou
View Article
humanWB

Sci Transl Med

An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs.

Authors - Wei-Jian Li
View Article
humanWB

Mol Cell

Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutants

Authors - Chao Yang
View Article
humanWB

Mol Cell

CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.

Authors - Ran Lin
  • KO Validated
View Article
mouseIHC

Autophagy

BNIP3-dependent mitophagy promotes cytosolic localization of LC3B and metabolic homeostasis in the liver.

Authors - Maya Z Springer
View Article
mouseIF

J Am Soc Nephrol

Hnf4a Is Required for the Development of Cdh6-Expressing Progenitors into Proximal Tubules in the Mouse Kidney.

Authors - Sierra S Marable
View Article