• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

ATAT1 Polyclonal antibody, PBS Only

ATAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 28828-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ATAT1 Polyclonal antibody (28828-1-AP)

    WB analysis using 28828-1-AP (same clone as 28828-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATAT1 Polyclonal antibody (28828-1-AP)

    WB analysis using 28828-1-AP (same clone as 28828-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using ATAT1 Polyclonal antibody (28828-1-AP)

    IHC staining of rat testis using 28828-1-AP (same clone as 28828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using ATAT1 Polyclonal antibody (28828-1-AP)

    IHC staining of rat testis using 28828-1-AP (same clone as 28828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ATAT1 Polyclonal antibody (28828-1-AP)

    IHC staining of mouse testis using 28828-1-AP (same clone as 28828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ATAT1 Polyclonal antibody (28828-1-AP)

    IHC staining of mouse testis using 28828-1-AP (same clone as 28828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28828-1-AP (C6orf134 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    IF Staining of A549 using 28828-1-AP (same clone as 28828-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    IF Staining of A549 using 28828-1-AP (same clone as 28828-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    IF Staining of A549 using 28828-1-AP (same clone as 28828-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    IF Staining of A549 using 28828-1-AP (same clone as 28828-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    FC experiment of A431 using 28828-1-AP (same clone as 28828-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human ATAT1 (28828-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28828-1-PBS targets ATAT1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATAT1 fusion protein Ag28757 相同性解析による交差性が予測される生物種
Full Name chromosome 6 open reading frame 134
Calculated molecular weight 47 kDa
Observed molecular weight42-45 kDa
GenBank accession numberNM_001031722
Gene symbol ATAT1
Gene ID (NCBI) 79969
RRIDAB_2881219
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C6orf134 is a reported alias name for the human gene ATAT1 coding alpha-tubulin N-acetyltransferase 1, and specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development. Western blot shows a band between 36 kDa and 45 kDa.