ATF5 Monoclonal antibody
ATF5 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human and More (3)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1E5B6
Cat no : 67066-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, A431 cells, HT-29 cells, MCF-7 cells, MDA-MB-231 cells, SK-BR-3 cells, T-47D cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
67066-1-Ig targets ATF5 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, chicken, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ATF5 fusion protein Ag7214 相同性解析による交差性が予測される生物種 |
| Full Name | activating transcription factor 5 |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC005174 |
| Gene symbol | ATF5 |
| Gene ID (NCBI) | 22809 |
| RRID | AB_2882376 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATF5, also named as Activating transcription factor 5, is a 282 amino acid protein, which belongs to the bZIP family. ATF5 is Widely expressed with higher expression levels in liver. ATF5 is actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, it translocates from the centrosome to the midbody (PMID: 26213385) . ATF5 as a transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically ATF5 is involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATF5 antibody 67066-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer. | ||
Toxicol Lett Bavachin induces liver injury and cell apoptosis by targeting Wnt/β-catenin/DRP1 signaling pathway mediated mitochondrial dysfunction | ||
FEBS Open Bio Knockdown of TMEM160 leads to an increase in reactive oxygen species generation and the induction of the mitochondrial unfolded protein response |
