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- KD/KO Validated
ATG12 Polyclonal antibody
ATG12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 11264-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | PC-3 cells, COLO 320 cells, NIH/3T3 cells, HeLa cells, HCT 116 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
11264-1-AP targets ATG12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATG12 fusion protein Ag1791 相同性解析による交差性が予測される生物種 |
| Full Name | ATG12 autophagy related 12 homolog (S. cerevisiae) |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 48-55 kDa |
| GenBank accession number | BC012266 |
| Gene symbol | ATG12 |
| Gene ID (NCBI) | 9140 |
| RRID | AB_10807096 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG12 antibody 11264-1-AP | Download protocol |
| IHC protocol for ATG12 antibody 11264-1-AP | Download protocol |
| IF protocol for ATG12 antibody 11264-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Enhancing autophagy and energy metabolism in the meniscus can delay the occurrence of PTOA in ACLT rat | ||
Biochem J Protease-Activated Receptor-2 promotes kidney tubular epithelial inflammation by inhibiting autophagy via the PI3K/Akt/mTOR signalling pathway. | ||
Biology (Basel) Autophagy Alters Bladder Angiogenesis and Improves Bladder Hyperactivity in the Pathogenesis of Ketamine-Induced Cystitis in a Rat Model. | ||
Int J Biochem Cell Biol Sphingosine kinase 1 protects renal tubular epithelial cells from renal fibrosis via induction of autophagy. | ||
Eur J Pharmacol Latcripin-7A from Lentinula edodes C91-3 induces apoptosis, autophagy, and cell cycle arrest at G1 phase in human gastric cancer cells via inhibiting PI3K/Akt/mTOR signaling. | ||
Pharmacology LncRNA DDX11-AS1 Promotes Chemoresistance through LIN28A-Mediated ATG12 mRNA Stabilization in Breast Cancer |
