Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-11264 targets ATG12 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATG12 fusion protein Ag1791 相同性解析による交差性が予測される生物種 |
| Full Name | ATG12 autophagy related 12 homolog (S. cerevisiae) |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 48-55 kDa |
| GenBank accession number | BC012266 |
| Gene Symbol | ATG12 |
| Gene ID (NCBI) | 9140 |
| RRID | AB_3083895 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen Affinity Purified |
| UNIPROT ID | O94817 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ATG12 antibody CL488-11264 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |