CoraLite® Plus 488-conjugated ATG16L1 Polyclonal antibody
ATG16L1 Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-29445
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-29445 targets ATG16L1 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATG16L1 fusion protein Ag31318 相同性解析による交差性が予測される生物種 |
| Full Name | ATG16 autophagy related 16-like 1 (S. cerevisiae) |
| Calculated molecular weight | 607 aa, 68 kDa |
| Observed molecular weight | 68-75 kDa |
| GenBank accession number | BC000061 |
| Gene symbol | ATG16L1 |
| Gene ID (NCBI) | 55054 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ATG16L1 antibody CL488-29445 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
