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  • Featured Product
  • KD/KO Validated

GABARAPL1 Polyclonal antibody

GABARAPL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11010-1-AP

Synonyms

GABA(A) receptor-associated protein-like 1, Early estrogen-regulated protein, ATG8L, ATG8, APG8L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GABARAPL1 Polyclonal antibody (11010-1-AP)

    WB analysis using 11010-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using GABARAPL1 Polyclonal antibody (11010-1-AP)

    WB analysis of human heart using 11010-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using GABARAPL1 Polyclonal antibody (11010-1-AP)

    WB analysis of mouse liver using 11010-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using GABARAPL1 Polyclonal antibody (11010-1-AP)

    WB analysis of rat brain using 11010-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GABARAPL1 Polyclonal antibody (11010-1-AP)

    WB analysis of HepG2 using 11010-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using GABARAPL1 Polyclonal antibody (11010-1-AP)

    IP experiment of mouse liver using 11010-1-AP

    IP result of anti-GABARAPL1 (IP:11010-1-AP, 3ug; Detection:11010-1-AP 1:500) with mouse liver tissue lysate 5000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GABARAPL1 Polyclonal antibody (11010-1-AP)

    IHC staining of mouse brain using 11010-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11010-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GABARAPL1 Polyclonal antibody (11010-1-AP)

    IHC staining of mouse brain using 11010-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GABARAPL1 Polyclonal antibody (11010-1-AP)

    IHC staining of mouse brain using 11010-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using GABARAPL1 Polyclonal antibody (11010-1-AP)

    IHC staining of human ovary tumor using 11010-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using GABARAPL1 Polyclonal antibody (11010-1-AP)

    IHC staining of human liver using 11010-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using GABARAPL1 Polyclonal antibody (11010-1-AP)

    IHC staining of human liver using 11010-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GABARAPL1 Polyclonal antibody (11010-1-AP)

    IF Staining of HepG2 using 11010-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using GABARAPL1-Specific antibody (11010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GABARAPL1 Polyclonal antibody (11010-1-AP)

    IF Staining of HeLa using 11010-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using GABARAPL1-Specific antibody (11010-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GABARAPL1 Polyclonal antibody (11010-1-AP)

    IF Staining of HepG2 using 11010-1-AP

    Immunofluorescent analysis of Chloroquine treated HepG2 cells using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, human heart tissue, mouse liver tissue, rat brain tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse brain tissue, human ovary tumor tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inChloroquine treated HeLa cells, Chloroquine treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11010-1-AP targets GABARAPL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, prawn
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABARAPL1 fusion protein Ag1473 相同性解析による交差性が予測される生物種
Full Name GABA(A) receptor-associated protein like 1
Calculated molecular weight 14 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC009309
Gene symbol GABARAPL1
Gene ID (NCBI) 23710
RRIDAB_2294415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle and at very low levels in the thymus and small intestine. The antibody is specific to GABARAPL1, and it doesn't recognize the recombinant GABARAP and GABARAPL2.

Protocols

Product Specific Protocols
WB protocol for GABARAPL1 antibody 11010-1-APDownload protocol
IHC protocol for GABARAPL1 antibody 11010-1-APDownload protocol
IF protocol for GABARAPL1 antibody 11010-1-APDownload protocol
IP protocol for GABARAPL1 antibody 11010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Circulation

Markers of autophagy are downregulated in failing human heart after mechanical unloading.

Authors - Kassiotis Christos C
View Article
humanWB,IF,IP

J Extracell Vesicles

Secretion of pro-angiogenic extracellular vesicles during hypoxia is dependent on the autophagy-related protein GABARAPL1.

Authors - Tom G Keulers
View Article
mouseWB

Cell Metab

FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells.

Authors - Zhao Jinghui J
View Article
mouseWB

Autophagy

Analysis of ATG4C function in vivo

Authors - Isaac Tamargo-Gómez
View Article
mouseWB,IF

J Clin Invest

Autophagy is essential for mouse sense of balance.

Authors - Mari?o Guillermo G
View Article
humanWB

Autophagy

GABARAPL1 antibodies: target one protein, get one free!

Authors - Le Grand Jaclyn Nicole JN
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Margaux (Verified Customer) (06-07-2024)

Efficient to immunoprecipitate GABARAPL1 but I used another antibody to see it because it didn't work for my western-blot in my experimental conditions.

  • Applications: Immunoprecipitation
FH

H (Verified Customer) (04-06-2020)

I could not detect GABARAP L1 in HEK293T and HCT116 cells. It is possible that the expression level of GABARAP L1 in these two cell lines are extremely low. In proteomics analysis of the HEK293T whole cell lysates, however, we could detect GABARAP L1.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T and HCT116
GABARAPL1 Antibody Western Blot validation (1:1000 dilution) in HEK293T and HCT116 (Cat no:11010-1-AP)