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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GABARAPL1-Specific Polyclonal antibody

GABARAPL1-Specific Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11010

Synonyms

APG8 LIKE, APG8L, ATG8, ATG8L, GABARAPL1, GABARAPL1-Specific, GEC 1, GEC1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated GABARAPL1-Specific P (CL488-11010)

    IF Staining of HepG2 using CL488-11010

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CoraLite® Plus 488 GABARAPL1-Specific antibody (CL488-11010) at dilution of 1:200, CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated GABARAPL1-Specific P (CL488-11010)

    IF Staining of HepG2 using CL488-11010

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CoraLite® Plus 488 GABARAPL1-Specific antibody (CL488-11010) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inChloroquine treated HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11010 targets GABARAPL1-Specific in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABARAPL1-Specific fusion protein Ag1473 相同性解析による交差性が予測される生物種
Full Name GABA(A) receptor-associated protein like 1
Calculated molecular weight 14 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC009309
Gene symbol GABARAPL1
Gene ID (NCBI) 23710
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle and at very low levels in thymus and small intestine. The antibody is specific to GABARAPL1, and it doesn't recognize the recombinant GABARAP and GABARAPL2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GABARAPL1-Specific antibody CL488-11010Download protocol
Standard Protocols
Click here to view our Standard Protocols