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ATL3 Monoclonal antibody

ATL3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E7C4

Cat no : 68516-1-Ig

Synonyms

EC:3.6.5.-, Atlastin-3, Atlastin 3, 2E7C4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATL3 Monoclonal antibody (68516-1-Ig)

    WB analysis using 68516-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68516-1-Ig (ATL3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ATL3 Monoclonal antibody (68516-1-Ig)

    WB analysis of Jurkat using 68516-1-Ig

    WB result of ATL3 antibody (68516-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATL3 transfected Jurkat cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATL3 Monoclonal antibody (68516-1-Ig)

    IF Staining of HeLa using 68516-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (68516-1-Ig, Clone: 2E7C4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

Positive WB detected inPC-3 cells, Jurkat cells, HeLa cells, K-562 cells, rabbit testis tissue, rat testis tissue, mouse testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68516-1-Ig targets ATL3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATL3 fusion protein Ag10248 相同性解析による交差性が予測される生物種
Full Name atlastin GTPase 3
Calculated molecular weight 541 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC077727
Gene symbol ATL3
Gene ID (NCBI) 25923
RRIDAB_3085225
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.

Protocols

Product Specific Protocols
WB protocol for ATL3 antibody 68516-1-IgDownload protocol
IF protocol for ATL3 antibody 68516-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols