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  • Featured Product
  • KD/KO Validated

ATL3 Monoclonal antibody, PBS Only (Detector)

ATL3 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E7C4

Cat no : 68516-1-PBS

Synonyms

EC:3.6.5.-, Atlastin-3, Atlastin 3, 2E7C4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATL3 Monoclonal antibody (68516-1-Ig)

    WB analysis using 68516-1-Ig (same clone as 68516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68516-1-Ig (ATL3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68516-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ATL3 Monoclonal antibody (68516-1-Ig)

    WB analysis of Jurkat using 68516-1-Ig (same clone as 68516-1-PBS)

    WB result of ATL3 antibody (68516-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATL3 transfected Jurkat cells. This data was developed using the same antibody clone with 68516-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50703-1

    Cytometric bead array standard curve of MP50703-1

    Cytometric bead array standard curve of MP50703-1, ATL3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68516-2-PBS. Detection antibody: 68516-1-PBS. Standard:Ag10248. Range: 0.781-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68516-1-PBS targets ATL3 as part of a matched antibody pair:

MP50703-1: 68516-2-PBS capture and 68516-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATL3 fusion protein Ag10248 相同性解析による交差性が予測される生物種
Full Name atlastin GTPase 3
Calculated molecular weight 541 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC077727
Gene symbol ATL3
Gene ID (NCBI) 25923
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.