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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP1A1/2 Monoclonal antibody (68505-1-Ig)

    WB analysis using 68505-1-Ig (same clone as 68505-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SK-BR-3 cells using ATP1A1/2 Monoclonal antibody (68505-1-Ig)

    WB analysis of SK-BR-3 using 68505-1-Ig (same clone as 68505-1-PBS)

    SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using ATP1A1/2 Monoclonal antibody (68505-1-Ig)

    WB analysis of rat kidney using 68505-1-Ig (same clone as 68505-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using ATP1A1/2 Monoclonal antibody (68505-1-Ig)

    WB analysis of pig brain using 68505-1-Ig (same clone as 68505-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using ATP1A1/2 Monoclonal antibody (68505-1-Ig)

    IF Staining of C2C12 using 68505-1-Ig (same clone as 68505-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using ATP1A1/2 antibody (68505-1-Ig, Clone: 3D8G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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ATP1A1/2 Monoclonal antibody, PBS Only

ATP1A1/2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68505-1-PBS
Clone No.3D8G1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ATP1A2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2, alpha 2 ATPase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68505-1-PBS targets ATP1A1/2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP1A1/2 fusion protein Ag10489 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
Calculated molecular weight 1020 aa, 112 kDa
Observed molecular weight 97-100 kDa
GenBank accession numberBC052271
Gene Symbol ATP1A2
Gene ID (NCBI) 477
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50993
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ATP1A1/2 is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody can recognize ATP1A1 and ATP1A2.