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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP1A1/2 Monoclonal antibody

ATP1A1/2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3D8G1

Cat no : 68505-1-Ig

Synonyms

ATP1A2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2, alpha 2 ATPase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP1A1/2 Monoclonal antibody (68505-1-Ig)

    WB analysis using 68505-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SK-BR-3 cells using ATP1A1/2 Monoclonal antibody (68505-1-Ig)

    WB analysis of SK-BR-3 using 68505-1-Ig

    SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using ATP1A1/2 Monoclonal antibody (68505-1-Ig)

    WB analysis of rat kidney using 68505-1-Ig

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using ATP1A1/2 Monoclonal antibody (68505-1-Ig)

    WB analysis of pig brain using 68505-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using ATP1A1/2 Monoclonal antibody (68505-1-Ig)

    IF Staining of C2C12 using 68505-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using ATP1A1/2 antibody (68505-1-Ig, Clone: 3D8G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inrat brain tissue, SK-BR-3 cells, rat kidney tissue, pig brain tissue, mouse brain tissue
Positive IF/ICC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68505-1-Ig targets ATP1A1/2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP1A1/2 fusion protein Ag10489 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
Calculated molecular weight 1020 aa, 112 kDa
Observed molecular weight 97-100 kDa
GenBank accession numberBC052271
Gene symbol ATP1A2
Gene ID (NCBI) 477
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP1A1/2 is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody can recognize ATP1A1 and ATP1A2.

Protocols

Product Specific Protocols
WB protocol for ATP1A1/2 antibody 68505-1-IgDownload protocol
IF protocol for ATP1A1/2 antibody 68505-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols