ATP1A1 Monoclonal antibody
ATP1A1 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1E9B7
Cat no : 68735-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | unboiled A431 cells, unboiled HepG2 cells |
Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68735-2-Ig targets ATP1A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | ATP1A1 fusion protein Ag5990 相同性解析による交差性が予測される生物種 |
Full Name | ATPase, Na+/K+ transporting, alpha 1 polypeptide |
Calculated molecular weight | 113 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | BC050359 |
Gene symbol | ATP1A1 |
Gene ID (NCBI) | 476 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.
Protocols
Product Specific Protocols | |
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WB protocol for ATP1A1 antibody 68735-2-Ig | Download protocol |
IHC protocol for ATP1A1 antibody 68735-2-Ig | Download protocol |
IF protocol for ATP1A1 antibody 68735-2-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |