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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP1A1 Monoclonal antibody, PBS Only (Detector)

ATP1A1 Monoclonal Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E9B7

Cat no : 68735-2-PBS

Synonyms

ATP1A1, Sodium pump subunit alpha 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP1A1 Monoclonal antibody (68735-2-Ig)

    WB analysis using 68735-2-Ig (same clone as 68735-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68735-2-Ig (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68735-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50088-1

    Sandwich ELISA standard curve of MP50088-1

    Sandwich ELISA standard curve of MP50088-1, ATP1A1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68735-1-PBS. Detection antibody: HRP-conjugated 68735-2-PBS. Standard:Ag5990. Range: 0.195-50 ng/mL.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP1A1 Monoclonal antibody (68735-2-Ig)

    IHC staining of mouse kidney using 68735-2-Ig (same clone as 68735-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68735-2-Ig (ATP1A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68735-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP1A1 Monoclonal antibody (68735-2-Ig)

    IHC staining of mouse kidney using 68735-2-Ig (same clone as 68735-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68735-2-Ig (ATP1A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68735-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ATP1A1 Monoclonal antibody (68735-2-Ig)

    IF Staining of HEK-293 using 68735-2-Ig (same clone as 68735-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP1A1 antibody (68735-2-Ig, Clone: 1E9B7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68735-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68735-2-PBS targets ATP1A1 as part of a matched antibody pair:

MP50088-1: 68735-1-PBS capture and 68735-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATP1A1 fusion protein Ag5990 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated molecular weight 113 kDa
GenBank accession numberBC050359
Gene symbol ATP1A1
Gene ID (NCBI) 476
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.