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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated ATP5C1 Monoclonal antibody (CL594-60284)

    IF Staining of HepG2 using CL594-60284

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 ATP5C1 antibody (CL594-60284, Clone: 1B5A2 ) at dilution of 1:50.

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CoraLite®594-conjugated ATP5C1 Monoclonal antibody

ATP5C1 Monoclonal Antibody for IF/ICC
Cat No. CL594-60284
Clone No.1B5A2

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Synonyms

ATP5C, ATP5C1, ATP5CL1, F ATPase gamma subunit

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60284 targets ATP5C1 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATP5C1 fusion protein Ag1343 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC000931
Gene Symbol ATP5C1
Gene ID (NCBI) 509
RRIDAB_2919912
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36542
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057).

Protocols

Product Specific Protocols
IF protocol for CL594 ATP5C1 antibody CL594-60284Download protocol
Standard Protocols
Click here to view our Standard Protocols