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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP5D Polyclonal antibody (14893-1-AP)

    WB analysis using 14893-1-AP (same clone as 14893-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 14893-1-AP (ATP5D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14893-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using ATP5D Polyclonal antibody (14893-1-AP)

    WB analysis of A549 using 14893-1-AP (same clone as 14893-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 14893-1-AP (ATP5D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14893-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using ATP5D Polyclonal antibody (14893-1-AP)

    WB analysis of NIH/3T3 using 14893-1-AP (same clone as 14893-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14893-1-AP (ATP5D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP5D Polyclonal antibody (14893-1-AP)

    IHC staining of human liver cancer using 14893-1-AP (same clone as 14893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP5D Polyclonal antibody (14893-1-AP)

    IHC staining of human liver cancer using 14893-1-AP (same clone as 14893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP5D Polyclonal antibody (14893-1-AP)

    IHC staining of human liver cancer using 14893-1-AP (same clone as 14893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP5D Polyclonal antibody (14893-1-AP)

    IHC staining of human kidney using 14893-1-AP (same clone as 14893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP5D Polyclonal antibody (14893-1-AP)

    IHC staining of human kidney using 14893-1-AP (same clone as 14893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ATP5D Polyclonal antibody (14893-1-AP)

    IHC staining of human lung cancer using 14893-1-AP (same clone as 14893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ATP5D Polyclonal antibody (14893-1-AP)

    IHC staining of human lung cancer using 14893-1-AP (same clone as 14893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ATP5D Polyclonal antibody (14893-1-AP)

    IHC staining of human liver cancer using 14893-1-AP (same clone as 14893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14893-1-PBS in a different storage buffer formulation.

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ATP5D Polyclonal antibody, PBS Only

ATP5D Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14893-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14893-1-PBS targets ATP5D in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP5D fusion protein Ag6682 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
Calculated molecular weight 17 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberBC002389
Gene Symbol ATP5D
Gene ID (NCBI) 513
RRIDAB_2274510
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30049
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The mitochondrial F1Fo-ATP synthase complex uses energy derived from a proton gradient to synthesize ATP. The ATP5D gene encodes the delta subunit of the mitochondrial ATP synthase F1 complex and belongs to the ATPase epsilon chain family. This gene encode the full length protein of 17 kDa with a transit peptide of 22 amino acid.