CoraLite® Plus 488-conjugated ATP5O Monoclonal antibody
ATP5O Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1D3B8
Cat no : CL488-66696
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66696 targets ATP5O in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ATP5O fusion protein Ag1458 相同性解析による交差性が予測される生物種 |
| Full Name | ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit |
| Calculated molecular weight | 23 kDa |
| Observed molecular weight | 23-25 kDa |
| GenBank accession number | BC021233 |
| Gene symbol | ATP5O |
| Gene ID (NCBI) | 539 |
| RRID | AB_2883370 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ATP5O antibody CL488-66696 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
