Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 18274-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 18274-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 18274-1-AP
IP result of anti-ATP6V0D1 (IP:18274-1-AP, 4ug; Detection:18274-1-AP 1:4000) with mouse testis tissue lysate 1120 ug.
IHC staining of human kidney using 18274-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 18274-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 18274-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18274-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 18274-1-AP
Immunofluorescent analysis of HeLa cells using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 18274-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V0D1 antibody (18274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ATP6V0D1 Polyclonal antibody

ATP6V0D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18274-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

V ATPase subunit d 1, V ATPase AC39 subunit, p39, ATP6DV, ATP6D

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (18)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, human placenta tissue, HeLa cells, mouse kidney tissue, mouse testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	mouse kidney tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 16 publications below
IHC	See 1 publications below
IF	See 4 publications below
IP	See 2 publications below

Product Information

18274-1-AP targets ATP6V0D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATP6V0D1 fusion protein Ag13002 相同性解析による交差性が予測される生物種
Full Name	ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
Calculated molecular weight	351 aa, 40 kDa
Observed molecular weight	37-41 kDa
GenBank accession number	BC008861
Gene Symbol	ATP6V0D1
Gene ID (NCBI)	9114
RRID	AB_2258877
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P61421
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

Protocols

Product Specific Protocols
WB protocol for ATP6V0D1 antibody 18274-1-AP	Download protocol
IHC protocol for ATP6V0D1 antibody 18274-1-AP	Download protocol
IF protocol for ATP6V0D1 antibody 18274-1-AP	Download protocol
IP protocol for ATP6V0D1 antibody 18274-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Nat Metab Time of exercise differentially impacts bone growth in mice Authors - Shaoling Yu View Article
human	WB	Autophagy Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency Authors - Fangyang Wang View Article
mouse	WB	Autophagy Disruption of the vacuolar-type H(+)-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes. Authors - Sandra Kissing View Article
human	WB	Mol Ther Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells. Authors - Benfu Zhong View Article
mouse	WB	Cell Mol Life Sci The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour. Authors - Enrico Castroflorio View Article
		Cells A Role for the V0 Sector of the V-ATPase in Neuroexocytosis: Exogenous V0d Blocks Complexin and SNARE Interactions with V0c Authors - Christian Lévêque View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18274-1-AP

WB analysis of human placenta using 18274-1-AP

IP experiment of mouse testis using 18274-1-AP

IHC staining of human kidney using 18274-1-AP

IHC staining of mouse kidney using 18274-1-AP

IHC staining of mouse kidney using 18274-1-AP

IHC staining of human kidney using 18274-1-AP

IF Staining of HeLa using 18274-1-AP

IF Staining of HeLa using 18274-1-AP

ATP6V0D1 Polyclonal antibody

ATP6V0D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Time of exercise differentially impacts bone growth in mice

Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency

Disruption of the vacuolar-type H(+)-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes.

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.

A Role for the V0 Sector of the V-ATPase in Neuroexocytosis: Exogenous V0d Blocks Complexin and SNARE Interactions with V0c