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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ATP6V0D1 Polyclonal antibody

ATP6V0D1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18274

Synonyms

32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated ATP6V0D1 Polyclonal (CL488-18274)

    IF Staining of HeLa using CL488-18274

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ATP6V0D1 antibody (CL488-18274) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18274 targets ATP6V0D1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V0D1 fusion protein Ag13002 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
Calculated molecular weight 351 aa, 40 kDa
Observed molecular weight37-41 kDa
GenBank accession numberBC008861
Gene symbol ATP6V0D1
Gene ID (NCBI) 9114
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ATP6V0D1 antibody CL488-18274Download protocol
Standard Protocols
Click here to view our Standard Protocols