ATP6V0D1 Monoclonal antibody
ATP6V0D1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1G7A12
Cat no : 68506-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, zebrafish tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:1200-1:4800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68506-1-Ig targets ATP6V0D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish samples.
Tested Reactivity | Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | ATP6V0D1 fusion protein Ag13012 相同性解析による交差性が予測される生物種 |
Full Name | ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1 |
Calculated molecular weight | 351 aa, 40 kDa |
Observed molecular weight | 37-41 kDa |
GenBank accession number | BC008861 |
Gene symbol | ATP6V0D1 |
Gene ID (NCBI) | 9114 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.
Protocols
Product Specific Protocols | |
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WB protocol for ATP6V0D1 antibody 68506-1-Ig | Download protocol |
IHC protocol for ATP6V0D1 antibody 68506-1-Ig | Download protocol |
IF protocol for ATP6V0D1 antibody 68506-1-Ig | Download protocol |
IP protocol for ATP6V0D1 antibody 68506-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |