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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP6V0D1 Monoclonal antibody (68506-1-Ig)

    WB analysis using 68506-1-Ig (same clone as 68506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATP6V0D1 Monoclonal antibody (68506-1-Ig)

    IP experiment of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    IP result of anti-ATP6V0D1 (IP:68506-1-Ig, 4ug; Detection:68506-1-Ig 1:3000) with HeLa cells lysate 1520 ug. This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V0D1 Monoclonal antibody (68506-1-Ig)

    IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V0D1 Monoclonal antibody (68506-1-Ig)

    IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP6V0D1 Monoclonal antibody (68506-1-Ig)

    IF Staining of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V0D1 antibody (68506-1-Ig, Clone: 1G7A12 ) at dilution of 1:2400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

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ATP6V0D1 Monoclonal antibody, PBS Only

ATP6V0D1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 68506-1-PBS
Clone No.1G7A12

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68506-1-PBS targets ATP6V0D1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP6V0D1 fusion protein Ag13012 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
Calculated molecular weight 351 aa, 40 kDa
Observed molecular weight37-41 kDa
GenBank accession numberBC008861
Gene Symbol ATP6V0D1
Gene ID (NCBI) 9114
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61421
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.