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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ATP6V1A Polyclonal antibody (51152-1-AP)

    WB analysis of HeLa using 51152-1-AP (same clone as 51152-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ATP6V1A Polyclonal antibody (51152-1-AP)

    WB analysis of HEK-293 using 51152-1-AP (same clone as 51152-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using ATP6V1A Polyclonal antibody (51152-1-AP)

    WB analysis of human brain using 51152-1-AP (same clone as 51152-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using ATP6V1A Polyclonal antibody (51152-1-AP)

    WB analysis of mouse kidney using 51152-1-AP (same clone as 51152-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ATP6V1A Polyclonal antibody (51152-1-AP)

    IHC staining of human testis using 51152-1-AP (same clone as 51152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51152-1-AP (ATP6V1A Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ATP6V1A Polyclonal antibody (51152-1-AP)

    IHC staining of human testis using 51152-1-AP (same clone as 51152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51152-1-AP (ATP6V1A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

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ATP6V1A Polyclonal antibody, PBS Only

ATP6V1A Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 51152-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51152-1-PBS targets ATP6V1A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberNM_001690
Gene Symbol ATP6V1A
Gene ID (NCBI) 523
RRIDAB_2881254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP38606
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.This antibody is specific to ATP6V1A.