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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP6V1A Monoclonal antibody (68440-1-Ig)

    WB analysis using 68440-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68440-1-Ig (ATP6V1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of HeLa cells using ATP6V1A Monoclonal antibody (68440-1-Ig)

    WB analysis of HeLa using 68440-1-Ig

    WB result of ATP6V1A antibody (68440-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1A transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V1A Monoclonal antibody (68440-1-Ig)

    IHC staining of mouse kidney using 68440-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V1A Monoclonal antibody (68440-1-Ig)

    IHC staining of mouse kidney using 68440-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using ATP6V1A Monoclonal antibody (68440-1-Ig)

    FC experiment of HeLa using 68440-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATP6V1A Monoclonal antibody (68440-1-Ig, Clone:1C5G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO Validated

ATP6V1A Monoclonal antibody

ATP6V1A Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68440-1-Ig
Clone No.1C5G2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Synonyms

V-ATPase 69 kDa subunit, VA68, EC:7.1.2.2, ATP6V1A1, ATP6A1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68440-1-Ig targets ATP6V1A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATP6V1A fusion protein Ag10820 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
Calculated molecular weight 617 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC013138
Gene Symbol ATP6V1A
Gene ID (NCBI) 523
RRIDAB_3085153
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP38606
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.

Protocols

Product Specific Protocols
WB protocol for ATP6V1A antibody 68440-1-IgDownload protocol
IHC protocol for ATP6V1A antibody 68440-1-IgDownload protocol
FC protocol for ATP6V1A antibody 68440-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols