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ATP6V1A Monoclonal antibody

ATP6V1A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C5G2

Cat no : 68440-1-Ig

Synonyms

ATP6A1, ATP6V1A, ATP6V1A1, HO68, V ATPase 69 kDa subunit, V ATPase subunit A, VA68, Vacuolar ATPase isoform VA68, Vma1, VPP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP6V1A Monoclonal antibody (68440-1-Ig)

    WB analysis using 68440-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68440-1-Ig (ATP6V1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of HeLa cells using ATP6V1A Monoclonal antibody (68440-1-Ig)

    WB analysis of HeLa using 68440-1-Ig

    WB result of ATP6V1A antibody (68440-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1A transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V1A Monoclonal antibody (68440-1-Ig)

    IHC staining of mouse kidney using 68440-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V1A Monoclonal antibody (68440-1-Ig)

    IHC staining of mouse kidney using 68440-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68440-1-Ig targets ATP6V1A in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATP6V1A fusion protein Ag10820 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
Calculated molecular weight 617 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC013138
Gene symbol ATP6V1A
Gene ID (NCBI) 523
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.

Protocols

Product Specific Protocols
WB protocol for ATP6V1A antibody 68440-1-IgDownload protocol
IHC protocol for ATP6V1A antibody 68440-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols