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  • Featured Product
  • KD/KO Validated

ATP6V1A Monoclonal antibody, PBS Only

ATP6V1A Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C5G2

Cat no : 68440-1-PBS

Synonyms

V-ATPase 69 kDa subunit, VA68, EC:7.1.2.2, ATP6V1A1, ATP6A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP6V1A Monoclonal antibody (68440-1-Ig)

    WB analysis using 68440-1-Ig (same clone as 68440-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68440-1-Ig (ATP6V1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ATP6V1A Monoclonal antibody (68440-1-Ig)

    WB analysis of HeLa using 68440-1-Ig (same clone as 68440-1-PBS)

    WB result of ATP6V1A antibody (68440-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1A transfected HeLa cells. This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V1A Monoclonal antibody (68440-1-Ig)

    IHC staining of mouse kidney using 68440-1-Ig (same clone as 68440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V1A Monoclonal antibody (68440-1-Ig)

    IHC staining of mouse kidney using 68440-1-Ig (same clone as 68440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68440-1-PBS targets ATP6V1A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATP6V1A fusion protein Ag10820 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
Calculated molecular weight 617 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC013138
Gene symbol ATP6V1A
Gene ID (NCBI) 523
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.