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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP6V1B1 Monoclonal antibody, PBS Only

ATP6V1B1 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10A8

Cat no : 68219-1-PBS

Synonyms

ATP6B1, ATP6V1B1, RTA1B, V ATPase subunit B 1, VATB, VMA2, VPP3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP6V1B1 Monoclonal antibody (68219-1-Ig)

    WB analysis using 68219-1-Ig (same clone as 68219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68219-1-Ig (ATP6V1B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68219-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ATP6V1B1 Monoclonal antibody (68219-1-Ig)

    IF Staining of HEK-293 using 68219-1-Ig (same clone as 68219-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP6V1B1 antibody (68219-1-Ig, Clone: 1G10A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68219-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68219-1-PBS targets ATP6V1B1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP6V1B1 fusion protein Ag7405 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Calculated molecular weight 57 kDa
Observed molecular weight56 kDa
GenBank accession numberBC063411
Gene symbol ATP6V1B1
Gene ID (NCBI) 525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.