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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP6V1B1 Monoclonal antibody (68219-1-Ig)

    WB analysis using 68219-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68219-1-Ig (ATP6V1B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ATP6V1B1 Monoclonal antibody (68219-1-Ig)

    IF Staining of HEK-293 using 68219-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP6V1B1 antibody (68219-1-Ig, Clone: 1G10A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ATP6V1B1 Monoclonal antibody

ATP6V1B1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68219-1-Ig
Clone No.1G10A8

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IF/ICC, ELISA

Synonyms

ATP6B1, ATP6V1B1, RTA1B, V ATPase subunit B 1, VATB, VMA2, VPP3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJAR cells, pig brain tissue, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue, rat kidney tissue, rat brain tissue, mouse kidney tissue, mouse brain tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68219-1-Ig targets ATP6V1B1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP6V1B1 fusion protein Ag7405 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Calculated molecular weight 57 kDa
Observed molecular weight56 kDa
GenBank accession numberBC063411
Gene Symbol ATP6V1B1
Gene ID (NCBI) 525
RRIDAB_2935307
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15313
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.

Protocols

Product Specific Protocols
WB protocol for ATP6V1B1 antibody 68219-1-IgDownload protocol
IF protocol for ATP6V1B1 antibody 68219-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols