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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ATP6V1B1 Monoclonal antibody

ATP6V1B1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G10A8

Cat no : CL488-68219

Synonyms

V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated ATP6V1B1 Monoclonal (CL488-68219)

    IF Staining of HEK-293 using CL488-68219

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 ATP6V1B1 antibody (CL488-68219, Clone: 1G10A8 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68219 targets ATP6V1B1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP6V1B1 fusion protein Ag7405 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Calculated molecular weight 57 kDa
Observed molecular weight56 kDa
GenBank accession numberBC063411
Gene symbol ATP6V1B1
Gene ID (NCBI) 525
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ATP6V1B1 antibody CL488-68219Download protocol
Standard Protocols
Click here to view our Standard Protocols