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ATP6V1B2 Monoclonal antibody

ATP6V1B2 Monoclonal Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

3C11A9

Cat no : 68441-1-Ig

Synonyms

V-ATPase subunit B 2, VATB, HO57, ATP6B2, ATP6B1B2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP6V1B2 Monoclonal antibody (68441-1-Ig)

    WB analysis using 68441-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68441-1-Ig (ATP6V1B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of A549 cells using ATP6V1B2 Monoclonal antibody (68441-1-Ig)

    WB analysis of A549 using 68441-1-Ig

    WB result of ATP6V1B2 antibody (68441-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1B2 transfected A549 cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATP6V1B2 Monoclonal antibody (68441-1-Ig)

    IP experiment of HeLa using 68441-1-Ig

    IP result of anti-ATP6V1B2 (IP:68441-1-Ig, 4ug; Detection:68441-1-Ig 1:4000) with HeLa cells lysate 2000 ug.

  • Flow cytometry (FC) experiment of HeLa cells using ATP6V1B2 Monoclonal antibody (68441-1-Ig)

    FC experiment of HeLa using 68441-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug ATP6V1B2 Monoclonal antibody (68441-1-Ig, Clone:3C11A9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, mouse brain tissue
Positive IP detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68441-1-Ig targets ATP6V1B2 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP6V1B2 fusion protein Ag7265 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
Calculated molecular weight 57 kDa
Observed molecular weight 56-58 kDa
GenBank accession numberBC003100
Gene symbol ATP6V1B2
Gene ID (NCBI) 526
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).

Protocols

Product Specific Protocols
WB protocol for ATP6V1B2 antibody 68441-1-IgDownload protocol
IP protocol for ATP6V1B2 antibody 68441-1-IgDownload protocol
FC protocol for ATP6V1B2 antibody 68441-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols