• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

ATP6V1B2 Monoclonal antibody, PBS Only

ATP6V1B2 Monoclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C11A9

Cat no : 68441-1-PBS

Synonyms

ATP6B1B2, ATP6B2, ATP6V1B2, HO57, V ATPase subunit B 2, VATB, Vma2, VPP3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ATP6V1B2 Monoclonal antibody (68441-1-Ig)

    WB analysis using 68441-1-Ig (same clone as 68441-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68441-1-Ig (ATP6V1B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using ATP6V1B2 Monoclonal antibody (68441-1-Ig)

    WB analysis of A549 using 68441-1-Ig (same clone as 68441-1-PBS)

    WB result of ATP6V1B2 antibody (68441-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1B2 transfected A549 cells. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATP6V1B2 Monoclonal antibody (68441-1-Ig)

    IP experiment of HeLa using 68441-1-Ig (same clone as 68441-1-PBS)

    IP result of anti-ATP6V1B2 (IP:68441-1-Ig, 4ug; Detection:68441-1-Ig 1:4000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68441-1-PBS targets ATP6V1B2 in WB, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP6V1B2 fusion protein Ag7265 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
Calculated molecular weight 57 kDa
Observed molecular weight 56-58 kDa
GenBank accession numberBC003100
Gene symbol ATP6V1B2
Gene ID (NCBI) 526
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).