CoraLite® Plus 488-conjugated ATP6V1H Monoclonal antibody
ATP6V1H Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig, rabbit
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3G7B5
Cat no : CL488-68425
Synonyms
Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68425 targets ATP6V1H in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ATP6V1H fusion protein Ag10130 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H |
| Calculated molecular weight | 483 aa, 56 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC025275 |
| Gene symbol | ATP6V1H |
| Gene ID (NCBI) | 51606 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ATP6V1H antibody CL488-68425 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
