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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse testis tissue using ATP8A2 Polyclonal antibody (31697-1-AP)

    IP experiment of mouse testis using 31697-1-AP (same clone as 31697-1-PBS)

    IP result of anti-ATP8A2 (IP:31697-1-AP, 4ug; Detection:31697-1-AP 1:600) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 31697-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ATP8A2 Polyclonal antibody (31697-1-AP)

    IHC staining of mouse brain using 31697-1-AP (same clone as 31697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31697-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ATP8A2 Polyclonal antibody (31697-1-AP)

    IHC staining of mouse brain using 31697-1-AP (same clone as 31697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31697-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ATP8A2 Polyclonal antibody (31697-1-AP)

    IHC staining of mouse testis using 31697-1-AP (same clone as 31697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31697-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ATP8A2 Polyclonal antibody (31697-1-AP)

    IHC staining of mouse testis using 31697-1-AP (same clone as 31697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31697-1-PBS in a different storage buffer formulation.

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ATP8A2 Polyclonal antibody, PBS Only

ATP8A2 Polyclonal Antibody for IHC, IP, Indirect ELISA
Cat No. 31697-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31697-1-PBS targets ATP8A2 in IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP8A2 fusion protein Ag36276 相同性解析による交差性が予測される生物種
Full Name ATPase, aminophospholipid transporter-like, class I, type 8A, member 2
Calculated molecular weight133kDa
Observed molecular weight129 kDa
GenBank accession numberNM_001313741
Gene Symbol ATP8A2
Gene ID (NCBI) 51761
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9NTI2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ATP8A2 is also known as ATPIB. ATP8A2 is a member of the P4‐ATPase subfamily of P‐type ATPases that actively flips phosphatidylserine (PS) and phosphatidylethanolamine (PE) across membranes to generate and maintain transmembrane phospholipid asymmetry, a property important in such cellular processes as vesicle trafficking, apoptosis, cytokinesis, neuron survival, blood coagulation, and phagocytosis. ATP8A2 consists of an N‐domain involved in binding ATP, a P‐domain that undergoes phosphorylation, an A‐domain that functions in the dephosphorylation of the phosphorylated intermediate, and an M‐domain consisting of 10 transmembrane segments that comprise the pathway for translocation of the lipid across the membrane. ATP8A2 is highly expressed in the brain, spinal cord, testis, and retina (PMID: 33565221, PMID: 33766557, PMID: 37635783).