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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATPAF2 Monoclonal antibody (68351-1-Ig)

    WB analysis using 68351-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68351-1-Ig (ATPAF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using ATPAF2 Monoclonal antibody (68351-1-Ig)

    IHC staining of mouse heart using 68351-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68351-1-Ig (ATPAF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using ATPAF2 Monoclonal antibody (68351-1-Ig)

    IHC staining of mouse heart using 68351-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68351-1-Ig (ATPAF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATPAF2 Monoclonal antibody (68351-1-Ig)

    IF Staining of HepG2 using 68351-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATPAF2 antibody (68351-1-Ig, Clone: 1H9A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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ATPAF2 Monoclonal antibody

ATPAF2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68351-1-Ig
Clone No.1H9A9

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ATP12, ATP12 homolog, ATP12p, ATPAF2, LP3663

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68351-1-Ig targets ATPAF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATPAF2 fusion protein Ag29065 相同性解析による交差性が予測される生物種
Full Name ATP synthase mitochondrial F1 complex assembly factor 2
Calculated molecular weight 289 aa, 33 kDa
Observed molecular weight~30 kDa
GenBank accession numberBC032126
Gene Symbol ATPAF2
Gene ID (NCBI) 91647
RRIDAB_3085074
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N5M1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) also name as ATP12 and ATP12p, is an essential house keeping protein. ATPAF2 is an assembly factor for the F1 component of mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. The calculated MW is 33 kDa, 68351-1-Ig can detect bands around 30 kDa and 50 kDa, the larger band may correspond to dimer or complex form. (PMID: 1826907, 11410595)

Protocols

Product Specific Protocols
WB protocol for ATPAF2 antibody 68351-1-IgDownload protocol
IHC protocol for ATPAF2 antibody 68351-1-IgDownload protocol
IF protocol for ATPAF2 antibody 68351-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols