Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
68351-1-PBS targets ATPAF2 as part of a matched antibody pair:
MP50625-1: 68351-1-PBS capture and 68351-2-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ATPAF2 fusion protein Ag29065 相同性解析による交差性が予測される生物種 |
| Full Name | ATP synthase mitochondrial F1 complex assembly factor 2 |
| Calculated molecular weight | 289 aa, 33 kDa |
| Observed molecular weight | ~30 kDa |
| GenBank accession number | BC032126 |
| Gene Symbol | ATPAF2 |
| Gene ID (NCBI) | 91647 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8N5M1 |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) also name as ATP12 and ATP12p, is an essential house keeping protein. ATPAF2 is an assembly factor for the F1 component of mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. The calculated MW is 33 kDa, 68351-1-Ig can detect bands around 30 kDa and 50 kDa, the larger band may correspond to dimer or complex form. (PMID: 1826907, 11410595)